Rapd Marker Based Genetic Diversity Analysis of Jujube (Zivphus Mauritiana Lam.)

Abstract

The research work was carried out during June 2012 to March 2013 to determine the genetic variability of 15 selected Ziziphus mauritiana Lam. germplasm collected from the southwestern region of Bangladesh. In the present study, molecular characterization was used to record different levels of variability concerning the morphological traits of the fruit. The 10-mer and 12-mer oligonucleotide primers were used in RAPD for amplification. Three primers, A01, A02 and S07 were able to detect the amplification and yielded a total of 144 band patterns of which 69.74% were polymorphic. The highest percent of polymorphic loci (77.78%) was observed for primer S07 and the lowest (60.00%) for primer A01. Results were analyzed by molecular algorithm UPGMA. Fifteen genotypes on the dendrogram were identified and divided into two major groups and subgroups on the basis of genetic characteristics and OP8 is most diverged in cluster 02.1. The range of genetic distance was observed 0.090 (GP10 vs. GP07) to 0.625 (GP09 vs. GP06). Based on the cluster analysis, the Z. mauritiana samples that were believed to have high morphological difference were grouped independently. The results suggested that RAPD is useful to measure the genetic diversity of jujube germplasm.