Khulna University Digital Repository

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Recent Submissions

Luteinizing Hormone (Lh) And Follicle Stimulating Hormone (Fsh) In Modulation Of Insulin Gene Expression For Healthy Egg Production In Female (A Review)
(Biotechnology and Genetic Engineering Discipline, Khulna University, 2018-05) Zohra, Fatima Tuz; Ashraf, Dr. Ayeshas; Nasif Alvi
Egg health is one of the cornerstone of healthy fertility. To shield of a safe pregnancy, healthy egg production is ineluctable. Reproduction and metabolism are tightly connected and reciprocally regulated. Insulin plays a role in regulating reproduction and perform as a major signal linking metabolism and reproductive status. Insulin acts as a co-gonadotropin at the level of the ovary which enhances steroidogenic responses to gonadotropins. Most of the insulin pathways are active in the ovary and their ability to interact with each other and also with FSH and LH signaling pathways enables insulin to exert direct modulating influences on ovarian function. In this review, a modulatory effect of insulin gene expression on follicle stimulating hormone (FSH) and luteinizing hormone (LH) has been shown that could have been playing role on healthy fertility. The process is regulated by Akt pathway which is the signal transduction pathway of living cell in the ovary; where key proteins are Phosphatidylinositol 3 kinase (PI3K) and protein kinase B (Akt). Insulin signaling through Akt and FOXO 1 can enhance FSH and LH stimulated steroidogenesis. FSH and LH acting via mTOR/ERK can enhance cell proliferation and protein synthesis. When insulin binds to its receptor, it activates b-subunit tyrosin kinase which enhance PI3K activation. Then DNA is synthesized through p21 Ras and MAPK. Actually, Akt can not directly activate mTOR, only Rheb can. Inside the cell the Rheb is inactivated or turned off by Tsc1 and Tsc2. Akt inhibits this Tsc timers, then Rheb is free which regulate mTOR. Inositol glycans are also generated by insulin signaling pathway which mediate steroidogenic effects of insulin action. On the other hand, there are some negative regulators which are used the maintenance of dormancy of primordial follicles like PTEN, Tsc1, Tsc2, 4EBP1 etc. So there is a harmonic relationship among insulin, FSH and LH which guides to fabricate fertile follicular progress as well as manufacture of healthy egg.
Quality Aspects of Frozen Shrimp Product in Gemini Seafood Industry Between New Moon and Full Moon
(Fisheries and Marine Resource Technology Discipline, Khulna University, 2009-01) Islam, Md. Baharul; Huq, Dr. Khandaker Anisul; Nasif Alvi
The present study was conducted to assess the quality of shrimp in processing industry. The study area was Gemini Seafood Industry and the study period was 6 (six) month. The study included identification of Critical Control Point (CCP) in the processing industry. There are four shrimp process lines in the processing industry. Among four process lines, the CCP of block frozen shrimp, semi IQF shrimp and IQF shrimp were received raw material. The left process line is cooked IQF shrimp and the CCP was received raw material and cooking. Another set of experiment was carried out microbiological test of shrimp (received raw shrimp, export graded shrimp and final product), water and SWAB. The microbiological test was done during full moon and new moon and compared the result. The SPC of shrimp at different sampling points were varied between O.854xlO^5 to 5.74xlO^5 cfu/g and 0.614xl0^5 to 4.8x10^5 cfu/g during full moon and new moon correspondingly. The TC of shrimp was varied between 7.80 to 40.20 MPN/g and 5.60 to 31.20 MPN/g in full moon and new moon respectively. The Fe of shrimp was varied between 00 to 62 MPN/g and 00 to 5.4 MPN/g in full moon and new moon respectively. Salmonella sp. and V. cholerae were not detected in any sample in different sampling points during full moon and new moon. The SPC of water varied between 3-7 cfu/ml and 3-6 cfu/ml in full moon and new moon respectively. The SPC of SWAB (workers hand) before and after working was varied between 20-30/cm^2 and 20-25/cm^2 for full Moon and new Moon respectively. The SPC of SWAB (SS table) before and after working was varied between 15-25 cfu/cm^2 and 15-20 cfu/cm^2. All the values that were gotten, were within the acceptable limit which proved highly hygienic condition of the industry
Length-Weight Relationship and Condition Factor of Mystus gulio and Odontamblyopus rubicundus from the South Western Region of Bangladesh
(Fisheries and Marine Resource Technology Discipline, Khulna University, 2009-12) Afsana, Dalia; Sarower, Dr. Md. Golam; Nasif Alvi
Length-weight relationship, condition factor and relative condition factor of Mystus gulio and Odontamblyopus rubicundus were studied for a period of six months from October 2008 to March 2009. The results of the present study show that the length-weight of M gulio was linearly related and the equation obtained for total length-body weight and standard length-body weight was BW = -68.46 + 7.24TL (r = 0.93) and BW = -65.72 + 9.02SL (r = 0.94), respectively. The correlation coefficient indicate that the relationship between length and weight of the fish was highly significant. In case of 0. rubicundus the equation obtained for total length-body weight and standard length-body weight was BW = -4.63 + 0.71 TL (r = 0.94) and BW = -4.37 + 0.86SL (r = 0.94), respectively. The mean value of the condition factor of M gulio observed was 10.15±0.96. The lowest value of condition factor was observed as 7.69 in the length class of 12-14 cm and the highest value was observed as 13.3 in the length class of 10-12 cm. The values of condition factor of 0. rubicundus varied between 1.61 to 3.4 with a mean value of ~.29±0 .31. The highest value of the condition factor was observed in the length class of 9-10 cm and the lowest value was observed in the length class of 14-15 cm. The relative condition factor of M gulio varied from 0.75 to 1.31. The highest and the lowest value was recorded in the length class of 10-12cm and 14-16 cm, respectively. The mean value was observed as 0.99±0.09. The mean relative condition factor of 0. rubicundus was observed as 1.0±0.12. The lowest value of the relative condition factor was recorded as 0.66 in the length class of 6-7 cm and the highest value was observed as 1.45 in the length class of 9-10 cm.
Availability of Antioxidant in Fishes of Different Feeding Habits Available in Bangladesh
(Fisheries and Marine Resource Technology Discipline, Khulna University, 2011-04) Hasan, Md. Abir; Shah, Dr. Md. Saifuddin; Nasif Alvi
Free radical scavenging activity in the muscle of fifteen species of different feeding habit fishes were evaluated using both qualitative and quantitative method. Thin layer Chromatography (TLC) method was used for qualitative analysis. DPPH scavenging analysis reveals IC 50 of about 1266.02 ug/ml (Rhinomugil corsula), 963.46 ug/ml (Hypophthalmichthys molitrixy; 267.70 ug/ml (Puntius tieto), 965.75 ug/ml (Labeo bata), 1678.96 ug/ml (Puntius gonionotus), 327.69 ug/ml (Channa striatus), 346.32 ug/ml (Channa punctatus) ,2138.52 ug/ml (Notopterus chitala), 1757.17 ug/ml (Notopterus notopterus) , 1170.29 ug/ml (Mystus aor), 600.89 ug/ml (Pampus chinensisi, 872.19 ug/ml (Scatophagus argus), 397.72 f..lglml (Anabas testudineus), 154.45 ug/ml (Ctenopharyhgodon idella) and 530.69 ug/ml (Mackerel sp). In both the qualitative and quantitative method, ascorbic acid was used as positive control. Generally, herbivore fishes are supposed to get high antioxidant component as they consume mainly plant material and plants are the main source of antioxidant. But, in this experiment, no strong relationship was found among the feeding habit of fish and antioxidant activity. Two herbivore, two carnivore and one omnivorous fishes showed high antioxidant activity in this test. Being very common and rich in antioxidant, Channa striatus, Puntius ticto, Anabus testudenius, Ctenopharyhgodon idella and Channa punctatus can be used in our daily diet.
Breeding Technique and Embryonic Development of Mud Crab, Scylla Serrata
(Fisheries and Marine Resource Technology Discipline, Khulna University, 2012-08) Aktar, Nilufa; Sarower, Dr. Md. Golam; Nasif Alvi
Scylla serrata, commonly referred to as mud crabs, is an important species, both as a food item for local consumption as well as a commercially exportable product. The natural resource of this species is gradually declining due to human intervention and environmental modification that made the species a threatened one. Bringing this species into culture system can be a strategy to prevent it from being dwindled in the nature. With this aim in view, this study was conducted to know the brood development, breeding techniques and embryonic development of the species followed to develop a successful hatchery technology. The experiment was conducted "Sundarban Golda Hatchery" in Khulna. Ten brood crabs were collected from Munshigong crab farm and the average weight of crab was 180g. During breeding at hatchery salinity, temperature and pH of water were 30 ppt, 26 to 28 °c and 7.5 to 7.6 respectively. Matured brood crabs were hatched within over night. From the study, the average fecundity of the specimens was counted approximately 10, 00,000 and the average hatching was 4000 per 8 individual, hatching rate was approximately 5%. The embryonic development was checked and only one stage named blastula was possible to observe because of high embryo mortality. During larval rearing, newly hatched zoea was also observed. As the species is being rapidly declined from the natural waters, attention must be taken for bringing it under culture system and for adhere package in the larvae production and hatchery management technologies are necessary. This results promoted and be deemed as initial in this regard necessitating alternatives for conservation of the species.